Radiobiologie

• Radiační poškození DNA, proteinů a jejich komplexů
• Buněčná radiobiologie
• Biologické účinky nabitých částic, XUV a UV
• Teoretické modelování fyzikálního a chemického stádia interakce záření s biologickým materiálem ve vodě, vývoj stochastického kódu RADAMOL
• Charakterizace radioterapeutických svazků
• Fragmentace primárních iontů

Výběr z publikační činnosti

• Jelínek Michaelidesová, A., Kundrát, P., Zahradníček, O., Danilová, I., Pachnerová Brabcová, K., Vachelová, J., Vilimovský, J., David, M., Vondráček, V., Davídková, M. First independent validation of the proton-boron capture therapy concept. Scientific Reports. 14, (2024).
• Souli, M. et al. Clustered DNA damage patterns after proton therapy beam irradiation using plasmid DNA. Int. J. Mol. Sci. 23 (2022).

• Kraus, K. M. et al. Potential morbidity reduction for lung stereotactic body radiation therapy using respiratory gating. Cancers (Basel). 13, (2021).
• Simonetto, C. et al. Estimating long-term health risks after breast cancer radiotherapy: merging evidence from low and high doses. Radiat. Environ. Biophys. 60, 459–474 (2021).
• Kundrát, P., Friedland, W., Ottolenghi, A. & Baiocco, G. Coupling Radiation Transport and Track-Structure Simulations: Strategy Based on Analytical Formulas Representing DNA Damage Yields. Front. Phys. 9, 518 (2021).
• Tran, H. N. et al. Assessment of DNA damage with an adapted independent reaction time approach implemented in Geant4-DNA for the simulation of diffusion-controlled reactions between radio-induced reactive species and a chromatin fiber. Med. Phys. 48, 890–901 (2021).
• Wochnik, A. et al. Out-of-field doses for scanning proton radiotherapy of shallowly located paediatric tumours-a comparison of range shifter and 3D printed compensator. Phys. Med. Biol. 66, (2021).
• Port, M. et al. Gene Expression Changes in Irradiated Baboons: A Summary and Interpretation of a Decade of Findings. Radiation Research 195, 501–521 (2021).
• Sala, L. et al. Folding DNA into origami nanostructures enhances resistance to ionizing radiation. Nanoscale 13, 11197–11203 (2021).
• Krátká, M. et al. Gamma radiation effects on diamond field-effect biosensors with fibroblasts and extracellular matrix. Colloids Surfaces B Biointerfaces 204, 111689 (2021).
• Borm, K. J. et al. Toxicity of internal mammary irradiation in breast cancer. Are concerns still justified in times of modern treatment techniques? Acta Oncol. 59, 1201–1209 (2020).
• Kundrát, P. et al. Analytical formulas representing track-structure simulations on DNA damage induced by protons and light ions at radiotherapy-relevant energies. Sci. Reports 2020 101 10, 1–11 (2020).
• Hampl, M. et al. Polarized Sonic Hedgehog Protein Localization and a Shift in the Expression of Region-Specific Molecules Is Associated With the Secondary Palate Development in the Veiled Chameleon. Front. Cell Dev. Biol. 8, 572 (2020).
• Kavková, M. et al. Coordinated labio-lingual asymmetries in dental and bone development create a symmetrical acrodont dentition. Sci. Rep. 10, (2020).
• Torrisi, L., Davídková, M., Havránek, V., Cutroneo, M. & Torrisi, A. Physical study of proton therapy at CANAM laboratory on medulloblastoma cell lines DAOY. Radiat. Eff. Defects Solids 175, 863–878 (2020).
• Ostheim, P. et al. Overcoming challenges in human saliva gene expression measurements. Sci. Rep. 10, 1–12 (2020).
• Henthorn, N. T. et al. Mapping the Future of Particle Radiobiology in Europe: The INSPIRE Project. Front. Phys. 8, 438 (2020).
• Cruz-Garcia, L. et al. In vivo validation of alternative FDXR transcripts in human blood in response to ionizing radiation. Int. J. Mol. Sci. 21, 1–18 (2020).
• Michaelidesová, A. et al. In vitro comparison of passive and active clinical proton beams. Int. J. Mol. Sci. 21, 1–15 (2020).
• Kundrát, P. et al. What anatomic features govern personal long-term health risks from breast cancer radiotherapy? Radiat. Prot. Dosimetry 186, 381–385 (2019).
• Friedland, W., Kundrát, P., Becker, J. & Eidemüller, M. Biophysical simulation tool PARTRAC: Modelling proton beams at therapy-relevant energies. Radiat. Prot. Dosimetry 186, 172–175 (2019).
• Romanenko, O. et al. Performance and application of heavy ion nuclear microbeam facility at the Nuclear Physics Institute in Řež, Czech Republic. Rev. Sci. Instrum. 90, 013701 (2019).
• Schuemann, J. et al. A New Standard DNA Damage (SDD) Data Format. Radiat. Res. 191, 76–92 (2019).
• Pachnerová Brabcová, K., Sihver, L., Ukraintsev, E., Štěpán, V. & Davídková, M. How detection of plasmid DNA fragmentation affects radiation strand break yields. Radiat. Prot. Dosimetry 183, 89–92 (2019).
• Ondrák, L. et al. Radioprotective effect of hydroxyl radical scavengers on prokaryotic and eukaryotic cells under various gamma irradiation conditions. Radiat. Prot. Dosimetry 186, 186–190 (2019).
• Pachnerová Brabcová, K. et al. Radiation-induced plasmid DNA damage: Effect of concentration and length. Radiat. Prot. Dosimetry 186, 168–171 (2019).
• Konířová, J. et al. Differentiation Induction as a Response to Irradiation in Neural Stem Cells In Vitro. Cancers (Basel). 11, 913 (2019).
• Michaelidesová, A. et al. Comparison of the radiation sensitivity of the breast cancer cell line MCF7 and adipose-derived stem cells. Radiat. Prot. Dosimetry 186, 155–158 (2019).
• Michaelidesová, A., Konířová, J., Bartůněk, P. & Zíková, M. Effects of radiation therapy on neural stem cells. Genes vol. 10 640 (2019).
• Tichy, A. et al. The first in vivo multiparametric comparison of different radiation exposure biomarkers in human blood. PLoS One 13, (2018).
• Vyšín, L. et al. Biological action in and out of the water window. Acta Phys. Pol. A 133, 236–238 (2018).
• Stolarczyk, L. et al. Dose distribution of secondary radiation in a water phantom for a proton pencil beam - EURADOS WG9 intercomparison exercise. Phys. Med. Biol. 63, (2018).
• Ježková, L. et al. Particles with similar LET values generate DNA breaks of different complexity and reparability: A high-resolution microscopy analysis of γH2AX/53BP1 foci. Nanoscale 10, 1162–1179 (2018).
• Pachnerová Brabcová, K., Sihver, L., Ukraintsev, E., Štěpán, V. & Davídková, M. Length computation of irradiated plasmid DNA molecules. Biointerphases 13, 061005 (2018).
• Vyšín, L. et al. Dose-Rate Effects in Breaking DNA Strands by Short Pulses of Extreme Ultraviolet Radiation. Radiat. Res. 189, 466–476 (2018).
• Šefl, M., Pachnerová Brabcová, K. & Štěpán, V. Dosimetry as a Catch in Radiobiology Experiments. Radiat. Res. 190, 404–411 (2018).
• Reimitz, D., Davídková, M., Mestek, O., Pinkas, J. & Kočišek, J. Radiomodifying effects of RAPTA C and CDDP on DNA strand break induction. Radiat. Phys. Chem. 141, 229–234 (2017).
• Vyšín, L. et al. Degradation of phospholipids under different types of irradiation and varying oxygen saturation. Radiat. Environ. Biophys. 56, 241–247 (2017).
• Michaelidesová, A. et al. Relative biological effectiveness in a proton spread-out Bragg peak formed by pencil beam scanning mode. Australas. Phys. Eng. Sci. Med. 40, 359–368 (2017).
• Štěpán, V. & Davídková, M. Understanding radiation damage on sub-cellular scale using RADAMOL simulation tool. Radiat. Phys. Chem. 128, 11–17 (2016).
• Adjei, D. et al. DNA strand breaks induced by soft X-ray pulses from a compact laser plasma source. Radiat. Phys. Chem. 120, 17–25 (2016).
• Krátká, M. et al. Gamma radiation effects on hydrogen-terminated nanocrystalline diamond bio-transistors. Diam. Relat. Mater. 63, 186–191 (2016).
• Stursa, J. et al. Mass production of fluorescent nanodiamonds with a narrow emission intensity distribution. Carbon N. Y. 96, 812–818 (2016).
• Marshall, T. I. et al. Investigating the Implications of a Variable RBE on Proton Dose Fractionation Across a Clinical Pencil Beam Scanned Spread-Out Bragg Peak. Int. J. Radiat. Oncol. Biol. Phys. 95, 70–77 (2016).
• Pachnerová Brabcová, K. et al. Contribution of indirect effects to clustered damage in DNA irradiated with protons. Radiat. Prot. Dosimetry 166, 44–48 (2015).
• Vyšín, L. et al. Proton-induced direct and indirect damage of plasmid DNA. Radiat. Environ. Biophys. 54, 343–352 (2015).
• Bernal, M. A. et al. A review of the Geant4-DNA very low energy extension of the Geant4 Monte Carlo simulation toolkit. Phys. Medica 31, 861–874 (2015).
• Nováková, E. et al. Breaking DNA strands by extreme-ultraviolet laser pulses in vacuum. Phys. Rev. E - Stat. Nonlinear, Soft Matter Phys. 91, 042718 (2015).
• Litvinchuk, A. V., Vachelová, J., Michaelidesová, A., Wagner, R. & Davídková, M. Dose-dependent micronuclei formation in normal human fibroblasts exposed to proton radiation. Radiat. Environ. Biophys. 54, 327–34 (2015).
• Falk, M. et al. Determining omics spatiotemporal dimensions using exciting new nanoscopy techniques to assess complex cell responses to DNA damage: Part B-structuromics. Crit. Rev. Eukaryot. Gene Expr. 24, 225–247 (2014).
• Falk, M. et al. Primary and secondary clustering of DSB repair foci and repair kinetics compared for X-rays, protons of different energies and high-LET 20Ne ions. J. Radiat. Res. 55, i79–i80 (2014).
• Karamitros, M. et al. Diffusion-controlled reactions modeling in Geant4-DNA. J. Comput. Phys. 274, 841–882 (2014).
• Ježková, L. et al. Function of chromatin structure and dynamics in DNA damage, repair and misrepair: γ-rays and protons in action. Appl. Radiat. Isot. 83, 128–136 (2014).
• Incerti, S. et al. Simulating radial dose of ion tracks in liquid water simulated with Geant4-DNA: A comparative study. Nucl. Instruments Methods Phys. Res. Sect. B Beam Interact. with Mater. Atoms 333, 92–98 (2014).
• Falk, M. et al. Chromatin differentiation of white blood cells decreases DSB damage induction, prevents functional assembly of repair foci, but has no influence on protrusion of heterochromatic DSBs into the low-dense chromatin. J. Radiat. Res. 55, i81–i82 (2014).
• Pachnerová Brabcová, K. et al. Clustered DNA damage on subcellular level: effect of scavengers. Radiat. Environ. Biophys. 53, 705–712 (2014).
• Štěpán, V. & Davídková, M. RADAMOL tool: Role of radiation quality and charge transfer in damage distribution along DNA oligomer Guest editors: Andrey V. Solov’yov, Nigel Mason, Paulo Limão-Vieira, Malgorzata Smialek-Telega. Eur. Phys. J. D 68, 1–7 (2014).
• Adjei, D. et al. Development of a compact laser-produced plasma soft X-ray source for radiobiology experiments. Nucl. Instruments Methods Phys. Res. Sect. B Beam Interact. with Mater. Atoms 364, 27–32 (2014).
• Jezkova, L. et al. Function of chromatin structure and dynamics in DNA damage, repair and misrepair: gamma-rays and protons in action. Appl. Radiat. Isot. 83 Pt B, 128–136 (2014).
• Falk, M. et al. Heterochromatinization associated with cell differentiation as a model to study DNA double strand break induction and repair in the context of higher-order chromatin structure. Appl. Radiat. Isot. 83, 177–185 (2014).
• Falk, M. et al. Determining Omics spatiotemporal dimensions using exciting new nanoscopy techniques to assess complex cell responses to DNA damage: part A--radiomics. Crit Rev Eukaryot Gene Expr 24, 205–223 (2014).
• Staaf, E. et al. Characterisation of a setup for mixed beam exposures of cells to 241Am alpha particles and X-rays. Radiat. Prot. Dosimetry 151, 570–579 (2012).
• Staaf, E. et al. Micronuclei in human peripheral blood lymphocytes exposed to mixed beams of X-rays and alpha particles. Radiat. Environ. Biophys. 51, 283–293 (2012).
• Nováková, E. et al. Damage to dry plasmid DNA induced by nanosecond XUV-laser pulses. in Damage to VUV, EUV, and X-ray Optics III vol. 8077 80770W (SPIE, 2011).
• Spotheim-Maurizot, M. & Davídková, M. Radiation damage to DNA in DNA-protein complexes. Mutat. Res. - Fundam. Mol. Mech. Mutagen. 711, 41–48 (2011).
• Francis, Z. et al. Molecular scale track structure simulations in liquid water using the Geant4-DNA Monte-Carlo processes. Appl. Radiat. Isot. 69, 220–226 (2011).